What is a HPLC / High-Performance Liquid Chromatography?

HPLC (High Performance Liquid Chromatography) is a method of separating and detecting each compound contained in a sample using the interaction between the column and the sample. Since it can be easily measured and trace components can be detected, it is used in various industries such as pharmaceuticals, biochemistry, foods and the environment. In addition, since the peak area of ​​HPLC has a proportional relationship with the sample concentration, it is possible to quantify the concentration of the components contained in the sample. Since the sample separation behavior in HPLC differs depending on the column and mobile phase, it is necessary to design appropriate analytical conditions.

Application of HPLC / High-Performance Liquid Chromatography

✅ Pharmaceutical analysis:
HPLC is used in pharmaceutical quality control to measure the concentration of active ingredients and detect impurities. It is particularly useful in stability testing and monitoring during the manufacturing process.
✅ Food testing:
HPLC is used to analyze additives and pesticide residues in food. For example, it is used to measure the concentration of preservatives or chemicals in food products.
✅ Environmental analysis:
HPLC is used in water quality testing and to analyze harmful substances in the air. It can detect trace amounts of hazardous chemicals in environmental samples.

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