What is a HPLC?

HPLC (High Performance Liquid Chromatography) is a method of separating and detecting each compound contained in a sample using the interaction between the column and the sample. Since it can be easily measured and trace components can be detected, it is used in various industries such as pharmaceuticals, biochemistry, foods and the environment. In addition, since the peak area of ​​HPLC has a proportional relationship with the sample concentration, it is possible to quantify the concentration of the components contained in the sample. Since the sample separation behavior in HPLC differs depending on the column and mobile phase, it is necessary to design appropriate analytical conditions.

Application of HPLC

HPLC (High Performance Liquid Chromatography) is an analytical method that separates each component in a sample using the interaction between the column and the sample. It is very easy to use and can be analyzed by injecting the sample solution directly or by placing the sample solution on an autosampler and batch processing. HPLC is used in various industries. For example, in the pharmaceutical field, it is used for analysis of trace impurities and active ingredients, in the food and beverage field, it is used for analysis of nutritional components, functional components, additives, residual pesticides, etc., and in biochemistry, it is related to proteins and nucleic acids. It is used for substance analysis.

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